Increased intracellular Ca2+ signaling caused by the antitumor agent helenalin and its analogues

Garth Powis, Alfred Gallegos, Robert T. Abraham, Curtis L. Ashendel, Leon H. Zalkow, Gerald B. Grindey, Rosanne Bonjouklian

Research output: Contribution to journalArticle

  • 14 Citations

Abstract

The antitumor sesquiterpene lactone helenalin, which is found in species of the plant genus Helenium, cause a marked potentiation of the increases in intracellular free Ca2+ concentration ([Ca2+]i) produced by mitogens such as vasopressin, bradykinin, and platelet-derived growth factor in Swiss mouse 3T3 fibroblasts. Removing external Ca2+ partly attenuated the increased [Ca2+]i response. caused by helenalin. The increased [Ca2+]i responses occurred at concentrations of helenalin that inhibited cell proliferation. At higher concentrations, helenalin inhibited the [Ca2+]i responses. No change in resting [Ca2+]i was caused by helenalin even at high concentrations. Other helenalin analogues also increased the [Ca2+]i response. Helenalin did not inhibit protein kinase C (PKC) and PKC appeared to play a minor role in the effects of helenalin on [Ca2+]i responses in intact cells. Studies with saponin-permeabilized HT-29 human colon carcinosarcoma cells indicated that helenalin caused an increased accumulation of Ca2+ into nonmitochondrial stores and that the potentiating effect of helenalin on mitogen-stimulated [Ca2+]i responses was due in part to an increase in the inositol-(1,4,5)-trisphosphate-mediated release of Ca2+ from these stores.

Original languageEnglish (US)
Pages (from-to)344-350
Number of pages7
JournalCancer Chemotherapy and Pharmacology
Volume34
Issue number4
DOIs
StatePublished - Jul 1994

Fingerprint

Mitogens
Antineoplastic Agents
Protein Kinase C
Carcinosarcoma
Inositol 1,4,5-Trisphosphate
Sesquiterpenes
Platelet-Derived Growth Factor
Saponins
Bradykinin
Lactones
Vasopressins
Colon
Fibroblasts
Cell Proliferation
Cell proliferation

Keywords

  • Helenalin
  • Intracellular Ca

ASJC Scopus subject areas

  • Pharmacology
  • Oncology
  • Cancer Research

Cite this

Powis, G., Gallegos, A., Abraham, R. T., Ashendel, C. L., Zalkow, L. H., Grindey, G. B., & Bonjouklian, R. (1994). Increased intracellular Ca2+ signaling caused by the antitumor agent helenalin and its analogues. Cancer Chemotherapy and Pharmacology, 34(4), 344-350. DOI: 10.1007/BF00686043

Increased intracellular Ca2+ signaling caused by the antitumor agent helenalin and its analogues. / Powis, Garth; Gallegos, Alfred; Abraham, Robert T.; Ashendel, Curtis L.; Zalkow, Leon H.; Grindey, Gerald B.; Bonjouklian, Rosanne.

In: Cancer Chemotherapy and Pharmacology, Vol. 34, No. 4, 07.1994, p. 344-350.

Research output: Contribution to journalArticle

Powis, G, Gallegos, A, Abraham, RT, Ashendel, CL, Zalkow, LH, Grindey, GB & Bonjouklian, R 1994, 'Increased intracellular Ca2+ signaling caused by the antitumor agent helenalin and its analogues' Cancer Chemotherapy and Pharmacology, vol 34, no. 4, pp. 344-350. DOI: 10.1007/BF00686043
Powis G, Gallegos A, Abraham RT, Ashendel CL, Zalkow LH, Grindey GB et al. Increased intracellular Ca2+ signaling caused by the antitumor agent helenalin and its analogues. Cancer Chemotherapy and Pharmacology. 1994 Jul;34(4):344-350. Available from, DOI: 10.1007/BF00686043

Powis, Garth; Gallegos, Alfred; Abraham, Robert T.; Ashendel, Curtis L.; Zalkow, Leon H.; Grindey, Gerald B.; Bonjouklian, Rosanne / Increased intracellular Ca2+ signaling caused by the antitumor agent helenalin and its analogues.

In: Cancer Chemotherapy and Pharmacology, Vol. 34, No. 4, 07.1994, p. 344-350.

Research output: Contribution to journalArticle

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